iGEM project feedback: Pet Hairball Solution
我們是BASIS-China iGEM團隊，致力于用合成生物學方法解決寵物毛球癥問題。核心方案為角蛋白酶降解毛發(fā)+AKG調節(jié)腸道環(huán)境，這份問卷用于收集同儕反饋，優(yōu)化項目設計與Human Practices，感謝你的寶貴意見！
We are BASIS-China, a high school iGEM team dedicated to solving pet hairball disease with synthetic biology. Our core solution uses keratinase to degrade hair combined with AKG to regulate the intestinal environment. This questionnaire collects peer feedback to help us optimize our project design and human practices. Thank you for your valuable input!
1. 您的iGEM隊伍/學校是 Your iGEM team/school
2. 您在團隊中的角色是 Your role in the team A.Wet Lab（濕實驗） B.Dry Lab（建模/編程） C.Human Practices（科普/調研） D.Wiki/宣傳（Wiki/Outreach） E.其他
3. 您對合成生物學在寵物/動物健康領域的了解程度是Your familiarity with synthetic biology in pet/animal health A.完全不了解（Not familiar at all） B.略有了解（Slightly familiar） C.比較了解（Moderately familiar） D.非常了解（Very familiar）
4. 您認為“寵物毛球癥”作為項目主題，是否有明確的現(xiàn)實痛點和應用價值？Do you think "pet hairball disease" as a project topic has clear real-world pain points and application value? A.完全沒有價值（No value at all） B.有一定價值，但痛點不夠突出（Some value, but the pain point is not prominent enough） C.價值明確，痛點真實且有解決必要（Clear value; the pain point is real and worth solving） D.價值很高，是很有潛力的應用方向（High value; it is a very promising application direction）
5. 您認為“寵物毛球癥”作為項目主題，是否有明確的現(xiàn)實痛點和應用價值？Do you think "pet hairball disease" as a project topic has clear real-world pain points and application value? A.完全沒有價值（No value at all） B.有一定價值，但痛點不夠突出（Some value, but the pain point is not prominent enough） C.價值明確，痛點真實且有解決必要（Clear value; the pain point is real and worth solving） D.價值很高，是很有潛力的應用方向（High value; it is a very promising application direction）
6. 您對AKG是什么是否理解？Do you know what is AKG ? A.完全不理解（Do not understand at all） B.大致理解，但有部分疑問（Roughly understand, but have some concerns） C.基本理解（Basically understand） D.非常清晰 （Very clear）
7. 您對“角蛋白酶降解毛發(fā)+AKG調節(jié)腸道環(huán)境”的核心方案是否理解？Do you understand our core solution of "keratinase degrading hair + AKG regulating the intestinal environment"? A.完全不理解（Do not understand at all） B.大致理解，但有部分疑問（Roughly understand, but have some questions） C.基本理解，邏輯通順（Basically understand; the logic is sound） D.非常清晰，設計合理（Very clear; the design is reasonable）
8. 您認為角蛋白酶在寵物腸道內的表達/遞送可能存在哪些技術挑戰(zhàn)？What technical challenges do you think may exist in the expression/delivery of keratinase in the pet's intestine? A.酶的表達量不足，無法有效降解毛發(fā)（Insufficient enzyme expression, unable to degrade hair effectively） B.酶在腸道內的穩(wěn)定性差，易被消化液分解（Poor enzyme stability in the intestine, easily broken down by digestive fluids） C.遞送系統(tǒng)（如工程菌/益生菌）的定植效率問題（Colonization efficiency issues of the delivery system, e.g., engineered bacteria/probiotics） D.對寵物腸道菌群的潛在影響（Potential impact on the pet's gut microbiota） E.沒有明顯挑戰(zhàn)，方案可行（No obvious challenges; the plan is feasible） F.其他
9. 請給我們的項目整體維度打分Please rate our project A. 很差 very bad B. 較差 bad C. 一般 fine D. 良好 good E. 非常優(yōu)秀 excellent
10. 您認為我們目前的寵物主人問卷設計，是否能有效收集用戶真實需求？Do you think our current pet owner questionnaire design can effectively collect real user needs? A.問題太淺，無法挖掘深層需求（Questions are too superficial to dig into deep needs） B.基本覆蓋了核心問題，但可以補充更多細節(jié)（Covers core questions, but more details can be added） C.設計合理，能有效反映用戶態(tài)度（Well-designed; effectively reflects user attitudes）
11. 您覺得在華南分享會上，我們的項目介紹/展示哪些部分最吸引您？哪些部分最需要改進？In the presentation, which parts of our project introduction caught your attention the most? Which parts need the most improvement?
12. 您認為我們的最需要優(yōu)先解決的問題是什么？What do you think is the problem that we need to solve most?
13. 對于AKG在調節(jié)腸道微環(huán)境、促進腸道蠕動的作用，您認為我們目前的設計是否有需要補充/優(yōu)化的地方？For the role of AKG in regulating the intestinal microenvironment and promoting intestinal peristalsis, do you think our current design needs to be supplemented/optimized?
14. 您是否擔心工程菌/重組蛋白在寵物體內的安全性、逃逸風險或環(huán)境影響？如果是，您建議我們如何應對？Are you concerned about the safety, escape risk, or environmental impact of engineered bacteria/recombinant proteins in pets? If so, how do you suggest we address these concerns?
15. 您認為我們目前的實驗驗證思路（酶活測定、模擬腸道實驗、動物實驗等）是否完整？還需要補充哪些關鍵實驗？Do you think our current experimental validation plan (enzyme activity assay, simulated gut experiments, animal testing, etc.) is complete? What key experiments do you think need to be added?